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Proceedings Paper

Verteporfin heterogeneity in pancreatic adenocarcinoma and the relationship to tumor vasculature and collagen distribution
Author(s): Phuong Vincent; Rui Xie; Michael Nieskoski; Kayla Marra; Jason Gunn; Brian W. Pogue
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Paper Abstract

Photodynamic therapy (PDT) has emerged as one promising treatment regimen for several cancer types, with a clinical trial ongoing in pancreatic adenocarcinoma (PDAC). PDT treatment efficacy mainly depends on the combination of light delivery, oxygen availability and photosensitizer uptake, each of which can be limited in pancreas cancer. Therefore, increasing drug uptake in the tumor would make an important impact on treatment outcome. This study was conducted to focus on the issue with drug resistance by examining the relationship between photosensitizer verteporfin and tissue parameters such as collagen and vascular patency. Verteporfin uptake in the tumors was assessed by fluorescence imaging while collagen content and patent vessel area fraction were quantified by evaluating Masson’s Trichrome and Lectin pathology staining images. Two tumor cell lines – AsPC-1 and BxPC-3 – were modeled in nude mice to investigate the impact of different tumor microenvironments. Experimental results highlighted the correlation between vascular patency and verteporfin uptake. Collagen content was found to be an independent factor within each tumor line, but a comparison across two tumor types suggested that collagen area of greater than 10% of tumor cross section reflected a lower verteporfin uptake. It was observed that whole-slice tumor quantifications have showcased some interesting trends which could be greatly enhanced and further supported by regional analysis.

Paper Details

Date Published: 12 February 2018
PDF: 6 pages
Proc. SPIE 10476, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII, 1047617 (12 February 2018); doi: 10.1117/12.2309291
Show Author Affiliations
Phuong Vincent, Thayer School of Engineering at Dartmouth (United States)
Rui Xie, Thayer School of Engineering at Dartmouth (United States)
Michael Nieskoski, Thayer School of Engineering at Dartmouth (United States)
Kayla Marra, Thayer School of Engineering at Dartmouth (United States)
Jason Gunn, Thayer School of Engineering at Dartmouth (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)


Published in SPIE Proceedings Vol. 10476:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII
David H. Kessel; Tayyaba Hasan, Editor(s)

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