Share Email Print
cover

Proceedings Paper • new

Insight into microenvironment of tumor on the microscopic level with a focus on cancer-associated fibroblasts
Author(s): Irina N. Druzhkova; Maria M. Lukina; Varvara Dudenkova; Lubov E. Shimolina; Elena V. Zagaynova; Marina V. Shirmanova
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Cancer-associated fibroblasts (CAFs) are one of the key determinants in the malignant progression of cancer. The subject of this research was metabolic reorganization of CAFs and their participation in collagen cross-linking process. The metabolic differences between normal fibroblasts and CAFs were elucidated using two-photon fluorescence lifetime imaging microscopy (FLIM). Collagen structure in 3D model was assessed using second harmonic generation (SHG) microscopy. We show increased metabolic activity of fibroblasts derived from patient’s colon tumor with a shift to more oxidative metabolism compare to dermal fibroblasts. The results of the study of collagen suggest that CAFs may contribute to the tumor progression through the facilitation of collagen alignment. In general, our findings support the idea of the strong association between cancer cells and fibroblasts and extensive involvement of CAFs in modulation of tumor microenvironment.

Paper Details

Date Published: 17 May 2018
PDF: 9 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106852R (17 May 2018); doi: 10.1117/12.2307096
Show Author Affiliations
Irina N. Druzhkova, Privolzhsky Research Medical Univ. (Russian Federation)
Maria M. Lukina, Privolzhsky Research Medical Univ. (Russian Federation)
Nizhny Novgorod State Univ. (Russian Federation)
Varvara Dudenkova, Privolzhsky Research Medical Univ. (Russian Federation)
Nizhny Novgorod State Univ. (Russian Federation)
Lubov E. Shimolina, Privolzhsky Research Medical Univ. (Russian Federation)
Nizhny Novgorod State Univ. (Russian Federation)
Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
Marina V. Shirmanova, Privolzhsky Research Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

© SPIE. Terms of Use
Back to Top