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Proceedings Paper

Plasmon-based determination of macromolecular interactions with membrane-encapsulated nanoparticles
Author(s): Joanna L. Richens; Jonathan P. Bramble; Jennifer Bain; Paul O'Shea
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Paper Abstract

Nanoparticles exhibit various optical properties arising from scattering and absorption due to polariton excitation. The resulting frequency and amplitude is dependent on several factors such as particle size, shape, and dielectric environment. By modifying the environment of the nanoparticle surface, in particular by encapsulating an individual nanoparticle within a membrane bilayer comprising defined phospholipids, these properties may be utilised to interrogate molecular interactions adjacent to the particle surface to useful levels of sensitivity. We describe the underlying rationale of these properties and characterise the preparation and behaviour of the nanoparticles. We indicate the potential this approach may have for sensing and screening in analytical biomolecular technology by demonstrating that it can be utilised to reveal the kinetics of the molecular interactions of membrane associated events. We also indicate that the technique may yield higherorder structural information of the macromolecule-membrane interactions in a highly sensitive manner and discuss the physical origins of these potentially more exotic phenomena.

Paper Details

Date Published: 15 May 2018
PDF: 16 pages
Proc. SPIE 10672, Nanophotonics VII, 1067208 (15 May 2018); doi: 10.1117/12.2306974
Show Author Affiliations
Joanna L. Richens, The Univ. of Nottingham (United Kingdom)
Jonathan P. Bramble, The Univ. of British Columbia (Canada)
Jennifer Bain, Univ. of Sheffield (United Kingdom)
Paul O'Shea, The Univ. of British Columbia (Canada)
Lancaster Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 10672:
Nanophotonics VII
David L. Andrews; Angus J. Bain; Jean-Michel Nunzi; Andreas Ostendorf, Editor(s)

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