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Proceedings Paper

Mueller microscopy of anisotropic scattering media: theory and experiments
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Paper Abstract

The combined approach including experiments (Mueller polarimetry) and theory (differential Mueller matrix formalism) for the studies of anisotropic scattering media was tested on the model system of human skin. Custom-build Mueller polarimetric microscope was used for the studies of histological cuts of full-thickness skin equivalents generated from epidermal keratinocytes forming a multilayered epidermis on top of collagen I hydrogel with dermal fibroblasts. The sets of fixed unstained tissue cuts of different thicknesses (5μm - 30μm) were measured in transmission configuration. The values of polarimetric (dichroism and retardance) and depolarization parameters were calculated by applying pixel-wise the logarithmic decomposition of Mueller matrices. The parabolic dependence of depolarization parameters and linear dependence of polarimetric parameters on thickness, as predicted by theory, was confirmed by measurements. It proves that phenomenological modeling of complex anisotropic scattering medium (e.g., biological tissue) may effectively disentangle the polarimetric and depolarizing properties of the system understudies and may be used for analysis and diagnostics of tissue.

Paper Details

Date Published: 24 May 2018
PDF: 8 pages
Proc. SPIE 10677, Unconventional Optical Imaging, 1067718 (24 May 2018); doi: 10.1117/12.2306943
Show Author Affiliations
Hee Ryung Lee, LPICM, CNRS, Ecole polytechnique, Univ. Paris-Saclay (France)
Thomas Sang Hyuk Yoo, LPICM, CNRS, Ecole polytechnique, Univ. Paris-Saclay (France)
Pengcheng Li, Tsinghua Univ. (China)
Christian Lotz, Universitätsklinikum Würzburg (Germany)
Florian Kai Groeber-Becker, Universitätsklinikum Würzburg (Germany)
Fraunhofer-Institut für Silicatforschung ISC (Germany)
Sofia Dembski, Universitätsklinikum Würzburg (Germany)
Fraunhofer-Institut für Silicatforschung ISC (Germany)
Enric Garcia-Caurel, LPICM, CNRS, Ecole polytechnique, Univ. Paris-Saclay (France)
Razvigor Ossikovski, LPICM, CNRS, Ecole polytechnique, Univ. Paris-Saclay (France)
Tatiana Novikova, LPICM, CNRS, Ecole polytechnique, Univ. Paris-Saclay (France)


Published in SPIE Proceedings Vol. 10677:
Unconventional Optical Imaging
Corinne Fournier; Marc P. Georges; Gabriel Popescu, Editor(s)

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