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Dry mass and average phase shift dynamics in HeLa cells subjected to low-dose photodynamic treatment
Author(s): A. V. Belashov; A. A. Zhikhoreva; T. N. Belyaeva; E. S. Kornilova; A. V. Salova; I. V. Semenova; O. S. Vasyutinskii
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Paper Abstract

The paper presents results on the response of living HeLa cells in vitro to low-dose photodynamic treatment with Radachlorin photosensitizer. Quantitative monitoring of variations of optical and morphological parameters of cells was performed by means of digital holographic microscopy and assisted with observations in confocal fluorescent microscope. The statistical analysis of the results obtained demonstrated significant morphological changes of cells along with invariable dry mass. The AO/EB standard test validated cell membrane integrity and demonstrated cells rounding and membrane blebbing. These data allow us to assume apoptosis as a major pathway of cell death activated in our experimental conditions.

Paper Details

Date Published: 17 May 2018
PDF: 7 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 1068505 (17 May 2018); doi: 10.1117/12.2306810
Show Author Affiliations
A. V. Belashov, Ioffe Institute (Russian Federation)
A. A. Zhikhoreva, Ioffe Institute (Russian Federation)
ITMO Univ. (Russian Federation)
T. N. Belyaeva, Institute of Cytology (Russian Federation)
E. S. Kornilova, Institute of Cytology (Russian Federation)
A. V. Salova, Institute of Cytology (Russian Federation)
I. V. Semenova, Ioffe Institute (Russian Federation)
O. S. Vasyutinskii, Ioffe Institute (Russian Federation)


Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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