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Vibrational spectroscopy of tissue-engineered structures based on proteins, chitosan, and carbon nanotube conjugates
Author(s): A. A. Polokhin; Yu. O. Fedorova; A. Yu Gerasimenko
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Paper Abstract

In this work, tissue-engineered structures based on a matrix of protein conjugates, chitosan and carbon nanotubes were prepared and studied. Bovine serum albumin (BSA), bovine collagen (BС) were used. Two types of single-walled carbon nanotubes (SWCNTs) were used to form a strong internal scaffold in a protein-chitosan matrix under the action of laser radiation. Tissue-engineered structures were created by means of layered deposition and laser evaporation of the initial aqueous dispersion from SWCNT, BSA, BC and chitosan succinate. As sources of laser radiation, a continuous diode laser with a wavelength of 810 nm and a pulsed fiber laser with a wavelength of 1064 nm and frequency of 80 kHz were used. Studies of tissue-engineered structures were carried out using vibrational spectroscopy methods (IR and Raman). The changes in the frequencies and intensities of the corresponding absorption bands and Raman lines of the amide group oscillations were analyzed. IR spectra of tissue-engineered structures demonstrated a high degree of binding of organic (protein, chitosan) and inorganic (SWCNT) components. The structure and defectiveness of the carbon nanotube scaffold were investigated in the Raman spectra.

Paper Details

Date Published: 17 May 2018
PDF: 8 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106853H (17 May 2018); doi: 10.1117/12.2306639
Show Author Affiliations
A. A. Polokhin, National Research Univ. of Electronic Technology (Russian Federation)
Yu. O. Fedorova, National Research Univ. of Electronic Technology (Russian Federation)
A. Yu Gerasimenko, National Research Univ. of Electronic Technology (Russian Federation)
I.M. Sechenov First Moscow State Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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