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Diffuse reflectance spectroscopy for determination of optical properties and chromophore concentrations of mice internal organs in the range of 350 nm to 1860 nm
Author(s): Marcelo Saito Nogueira; Michael Raju; Jacqueline Gunther; Konstantin Grygoryev; Katarzyna Komolibus; Huihui Lu; Stefan Andersson-Engels
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Paper Abstract

The development of photomedical modalities for diagnostics and treatment has created a need for knowledge of the optical properties of the targeted biological tissues. These properties are essential to plan certain procedures, since they determine the light absorption, propagation and penetration in tissues. One way to measure these properties is based on diffuse reflectance spectroscopy (DRS). DRS can provide light absorption and scattering coefficients for each wavelength through a non-invasive, fast and in situ interrogation, and thereby tissue biochemical information. In this study, reflectance measurements of ex vivo mice organs were investigated in a wavelength range between 350 and 1860 nm. To the best of our knowledge, this range is broader than previous studies reported in the literature and is useful to study additional chromophores with absorption in the extended wavelength range. Also, it may provide a more accurate concentration of tissue chromophores when fitting the reflectance spectrum in this extended range. In order to extract these concentrations, optical properties were calculated in a wide spectral range through a fitting routine based on an inverse Monte-Carlo look-up table model. Measurements variability was assessed by calculating the Pearson correlation coefficients between each pair of measured spectra of the same type of organ.

Paper Details

Date Published: 17 May 2018
PDF: 20 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106853G (17 May 2018); doi: 10.1117/12.2306636
Show Author Affiliations
Marcelo Saito Nogueira, Tyndall National Institute (Ireland)
Univ. College Cork (Ireland)
Michael Raju, Tyndall National Institute (Ireland)
Univ. College Cork (Ireland)
Jacqueline Gunther, Tyndall National Institute (Ireland)
Konstantin Grygoryev, Tyndall National Institute (Ireland)
Katarzyna Komolibus, Tyndall National Institute (Ireland)
Huihui Lu, Tyndall National Institute (Ireland)
Stefan Andersson-Engels, Tyndall National Institute (Ireland)


Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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