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Proceedings Paper

Combined label-free/fluorescence platform based on Bloch surface waves biochips for cancer biomarker detection
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Paper Abstract

A biosensor platform based on Bloch Surface Waves and operating in angular interrogation mode is applied to the detection of a clinical biomarker (HER2-neu/ERBB2) related to breast cancer initiation/progression. Preparing regions for specific recognition of different proteins as well as a reference on the biochip enables to correct the signal for nonspecific effects. Additionally, label-free analysis and surface wave enhanced fluorescence detection can be applied and compared directly on the platform. Cell lysates with high and low expression levels of ERBB2 are analyzed. Comparing the signals of such ERBB2 positive and negative samples estimates the limit of detection at 1.7 ng/mL. This is well below the threshold of 15 ng/mL set by the FDA for clinically useful ERBB2 detection in human serum, demonstrating that 1DPC-based biochips are attractive candidates for breast cancer detection/monitoring.

Paper Details

Date Published: 17 May 2018
PDF: 9 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106850X (17 May 2018); doi: 10.1117/12.2306424
Show Author Affiliations
Norbert Danz, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Alberto Sinibaldi, Sapienza Univ. di Roma (Italy)
Matteo Allegretti, Instituto Nazionale Tumori Regina Elena (Italy)
Elisabetta Sepe, Sapienza Univ. di Roma (Italy)
Peter Munzert, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Patrizio Giacomini, Instituto Nazionale Tumori Regina Elena (Italy)
Francesco Michelotti, Sapienza Univ. di Roma (Italy)


Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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