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Proceedings Paper

Achieving super-resolution fluorescence imaging with focused spot illumination and non-negative inversions (Conference Presentation)
Author(s): Jiun-Yann Yu; Stephen R Becker; James Folberth; Bruce F Wallin; Simeng Chen; V V Narumanchi; Jian Xing; Carol J Cogswell
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Paper Abstract

We demonstrate a new method for two-dimensional super-resolution fluorescence imaging. Our method achieves more than 2-fold resolution enhancement over the Rayleigh resolution limit simply with a focused illumination spot and non-negative least-squares inversions, and with practical photon budgets that can be supported by common fluorophores used for biological fluorescence microscopy.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049907 (14 March 2018); doi: 10.1117/12.2306354
Show Author Affiliations
Jiun-Yann Yu, University of Colorado Boulder (United States)
Stephen R Becker, University of Colorado Boulder (United States)
James Folberth, University of Colorado Boulder (United States)
Bruce F Wallin, University of Colorado Boulder (United States)
Simeng Chen, University of Colorado Boulder (United States)
V V Narumanchi, University of Colorado Boulder (United States)
Jian Xing, University of Colorado Boulder (United States)
Carol J Cogswell, University of Colorado Boulder (United States)


Published in SPIE Proceedings Vol. 10499:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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