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Proceedings Paper

ZnO nanoflakes-based mediator free flexible biosensors for the selective detection of ethylglucuronide (EtG) and lactate
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Paper Abstract

We report on highly sensitive and flexible biosensors for noninvasive lactate and alcohol monitoring in human perspiration based on zinc oxide (ZnO) nanostructures that does not require linker layer for surface functionalization due to the high isoelectric point of ZnO. Towards fabrication of the biosensors, two-dimensional (2D) ZnO nanoflakes (NFs) were synthesized on flexible polyethylene terephthalate (PET) substrates employing single step sonochemical method after which lactate oxidase (LOx) and anti-body for ethyl glucuronide (EtG)-a metabolite of ethanol were immobilized atop without a linker layer. The cyclic voltammetry (CV) measurements in the concentration range of 10pM-10μM for lactate and 4.5 μM-0.45 M for EtG yielded minimum limit of detection of 10 pM and 4.5 μM, respectively for the electrode area of 0.5 × 0.5 cm2. Moreover, lactate sensor with ZnO NF electrodes demonstrated four times higher sensitivity compared to the ones with gold electrode that required DTSP linker layer for surface functionalization. High isoelectric point allows a direct, stable pathway for rapid electron transport without any mediator when an analyte is immobilized on NFs and improves electron transfer rate.

Paper Details

Date Published: 8 May 2018
PDF: 6 pages
Proc. SPIE 10639, Micro- and Nanotechnology Sensors, Systems, and Applications X, 106392O (8 May 2018); doi: 10.1117/12.2306001
Show Author Affiliations
Fahmida Alam, Florida International Univ. (United States)
Ahmed Hasnain Jalal, Florida International Univ. (United States)
Raju Sinha, Florida International Univ. (United States)
Yogeswaran Umasankar, Florida International Univ. (United States)
Shekhar Bhansali, Florida International Univ. (United States)
Nezih Pala, Florida International Univ. (United States)

Published in SPIE Proceedings Vol. 10639:
Micro- and Nanotechnology Sensors, Systems, and Applications X
Thomas George; Achyut K. Dutta; M. Saif Islam, Editor(s)

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