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Label-free bacteria detection using controllable microporous polyelectrolyte coated long-period fiber gratings (Conference Presentation)
Author(s): Fan Yang; Tzu-Lan Chang; Henry Du; Junfeng Liang; Fei Tian

Paper Abstract

A novel application of a highly sensitive biosensor based on long-period fiber gratings (LPFG) coated with microporous polyelectrolyte coating for Gram-negative bacteria detection was investigated. The uniform microporous coating with large surface area was fabricated with weak polyelectrolytes poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) via layer-by-layer (LbL) assembly, followed by a brief exposure to acidic aqueous solutions at pH=4. The diameter of the micropores could be adjusted in a wide range by thermal treatment and ionic etching. The effect of pore size and surface topography on bacteria adhesion was examinged. Optofluidic LPFG platform for real-time monitoring of the bacteria binding/ adhesion in a flowing condition was investigated by measuring the spectral shift in the resonance wavelength. The coated LPFG platform was further functionalized with covalently immobilized bacteria antibody for specific bacterial detection with a concentration as low as 104 CFU/ml. Comparing with the widely used biosensors based on surface plasmon resonance (SPR), no moving part or metal deposition is required in our biosensor, making it highly sensitive, accurate, compact and cost effective.

Paper Details

Date Published: 15 May 2018
PDF
Proc. SPIE 10662, Smart Biomedical and Physiological Sensor Technology XV, 106620J (15 May 2018); doi: 10.1117/12.2304843
Show Author Affiliations
Fan Yang, Stevens Institute of Technology (United States)
Tzu-Lan Chang, Stevens Institute of Technology (United States)
Henry Du, Stevens Institute of Technology (United States)
Junfeng Liang, Stevens Institute of Technology (United States)
Fei Tian, Stevens Institute of Technology (United States)


Published in SPIE Proceedings Vol. 10662:
Smart Biomedical and Physiological Sensor Technology XV
Brian M. Cullum; Douglas Kiehl; Eric S. McLamore, Editor(s)

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