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Improvement of the multiphoton fluorescence microscopy images quality using digital filtration
Author(s): Y. V. Kistenev; V. V. Nikolaev; A. K. Drozdova; E. E. Ilyasova; E. A. Sandykova
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Paper Abstract

In our study we used rank-order filter, the emissions filter on the base of the criteria of Pearson, Gaussian filter and median filterfor improving the is fluorescence lifetime imaging microscopy (FLIM) data. The data obtained with the FLIM technology are the distribution with a pronounced peak, while during measurement the peak value is measured with an error. According to the analysisthe Gaussian filter is more useful to improve quality of FLIM data.Spatial filtering allows to reduce the noise component, obtained in the course of measurements, including reduction the influence of the individual bursts. Filtering in time scale allows to determine a peak value of intensity more accurately.This research was carried out using the equipment of Tomsk Regional Common Use Center of Tomsk State University.

Paper Details

Date Published: 16 April 2018
PDF: 6 pages
Proc. SPIE 10614, International Conference on Atomic and Molecular Pulsed Lasers XIII, 106141Q (16 April 2018); doi: 10.1117/12.2303643
Show Author Affiliations
Y. V. Kistenev, Tomsk State Univ. (Russian Federation)
Siberian State Medical Univ. (Russian Federation)
V. V. Nikolaev, Tomsk State Univ. (Russian Federation)
A. K. Drozdova, Tomsk State Univ. (Russian Federation)
Siberian State Medical Univ. (Russian Federation)
E. E. Ilyasova, Tomsk State Univ. (Russian Federation)
Siberian State Medical Univ. (Russian Federation)
E. A. Sandykova, Tomsk State Univ. (Russian Federation)
Siberian State Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 10614:
International Conference on Atomic and Molecular Pulsed Lasers XIII
Andrei M. Kabanov; Victor F. Tarasenko, Editor(s)

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