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Proceedings Paper

Laser-activated protein bands for peripheral nerve repair
Author(s): Antonio Lauto; Rodney Ian Trickett; Richard Malik; Judith M. Dawes; Earl R. Owen
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Paper Abstract

A 100 micrometer core optical fiber-coupled 75 mW diode laser operating at a wavelength of 800 nm has been used in conjunction with a protein solder to stripe weld severed rat tibial nerves, reducing the long operating time required for microsurgical nerve repair. Welding is produced by selective laser denaturation of the protein based solder which contains the dye indocyanine green. Operating time for laser soldering was 10 plus or minus 5 min. (n equals 24) compared to 23 plus or minus 9 min (n equals 13) for microsuturing. The laser solder technique resulted in patent welds with a tensile strength of 15 plus or minus 5 g, while microsutured nerves had a tensile strength of 40 plus or minus 10 g. Histopathology of the laser soldered nerves, conducted immediately after surgery, displayed solder adhesion to the outer membrane with minimal damage to the inner axons of the nerves. An in vivo study, with a total of fifty-seven adult male wistar rats, compared laser solder repaired tibial nerves to conventional microsuture repair. Twenty-four laser soldered nerves and thirteen sutured nerves were characterized at three months and showed successful regeneration with average compound muscle action potentials (CMAP) of 2.4 plus or minus 0.7 mV and 2.7 plus or minus 0.8 mV respectively. Histopathology of the in vivo study, confirmed the comparable regeneration of axons in laser and suture operated nerves. A faster, less damaging and long lasting laser based anastomotic technique is presented.

Paper Details

Date Published: 19 January 1996
PDF: 10 pages
Proc. SPIE 2623, Medical Applications of Lasers III, (19 January 1996); doi: 10.1117/12.230350
Show Author Affiliations
Antonio Lauto, Macquarie Univ. (Australia)
Rodney Ian Trickett, Microsearch Foundation of Australia (Australia)
Richard Malik, Univ. of Sydney (Australia)
Judith M. Dawes, Macquarie Univ. (Australia)
Earl R. Owen, Microsearch Foundation of Australia (Australia)

Published in SPIE Proceedings Vol. 2623:
Medical Applications of Lasers III
Stephen G. Bown; Herbert J. Geschwind; Raimund Hibst; Frederic Laffitte; Giulio Maira; Roberto Pini; Hans-Dieter Reidenbach; Hans H. Scherer; Pasquale Spinelli, Editor(s)

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