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Proceedings Paper

Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture
Author(s): Josepa Rigau; Chung-Ho Sun; Mario A. Trelles; Michael W. Berns
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Paper Abstract

We previously described the influence of low-level laser therapy (LLLT) on the primary fibroblast ATCC CRL1471 CCD-19SK passage 7 in culture, metabolic changes and statistical significance absorption of 3[H]Hydroxyproline after 2 irradiations (12 hour intervals) with Ar:DYE Laser, 633 nm wavelength, output power 38 mw, spot size 3.5 cm, power density 4 mw/cm2, energy density plus or minus 2 J/cm2. The aim of this work is to investigate, by using the same procedure, the behavior of the confluence monolayer fibroblasts culture when a central scratch of 0.4 - 1 mm and 2 irradiations is performed, by means of the study of the colony formation, haptotaxis (direction) and chemotaxis- chemokinesis (movement). The results indicate that all these phenomena appear sooner in the LLLT cultures than in non-treated cultures. Thus we can confirm that LLLT induces fibroblast biological effects.

Paper Details

Date Published: 15 January 1996
PDF: 5 pages
Proc. SPIE 2630, Effects of Low-Power Light on Biological Systems, (15 January 1996); doi: 10.1117/12.230027
Show Author Affiliations
Josepa Rigau, Instituto Medico Vilafortuny (Spain)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Mario A. Trelles, Instituto Medico Vilafortuny (Spain)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)

Published in SPIE Proceedings Vol. 2630:
Effects of Low-Power Light on Biological Systems
Tiina I. Karu; Anthony R. Young, Editor(s)

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