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Proceedings Paper

UVA-induced oxidative stress in single cells probed by autofluorescence modifications, cloning assay, and comet assay
Author(s): Karsten Koenig; Tatiana B. Krasieva; Eckhard Bauer; Ulrich Fiedler; Michael W. Berns; Bruce J. Tromberg; Karl-Otto Greulich
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Paper Abstract

Cell damage by low-power 365 nm radiation of a 50 W high-pressure mercury microscopy lamp was studied. UVA exposure to CHO cells resulted for radiant exposures greater than 10 kJ/m2 in significant modifications of NADH-attributed autofluorescence and in inhibition of cell division. Single cell gel electrophoresis (comet assay) revealed UVA-induced single strand DNA breaks. According to these results, UVA excitation radiation in fluorescence microscopy may damage cells. This has to be considered in vital cell microscopy, e.g. in calcium measurements.

Paper Details

Date Published: 15 January 1996
PDF: 8 pages
Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); doi: 10.1117/12.230020
Show Author Affiliations
Karsten Koenig, Institut fuer Molekulare Biotechnologie eV (Germany)
Beckman Laser Institute and Medical Clinic (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Eckhard Bauer, Institut fuer Molekulare Biotechnologie eV (Germany)
Ulrich Fiedler, Institut fuer Molekulare Biotechnologie eV (Germany)
Michael W. Berns, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Karl-Otto Greulich, Institut fuer Molekulare Biotechnologie eV (Germany)


Published in SPIE Proceedings Vol. 2628:
Optical and Imaging Techniques for Biomonitoring
Hans-Jochen Foth; Renato Marchesini; Halina Podbielska; Michel Robert-Nicoud; Herbert Schneckenburger, Editor(s)

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