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Proceedings Paper

Two-photon excited cellular autofluorescence induced by cw and femtosecond NIR microradiation
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Paper Abstract

We report the first two-photon excited autofluorescence measurements in single cells induced by continuous wave NIR laser microbeams. In particular we demonstrate NIR-excited NAD(P)H fluorescence of motile spermatozoa in a single-beam gradient force optical trap ('optical tweezers'). In addition, two-photon NIR excited autofluorescence imaging and 'tau- mapping' of CHO cells was performed with laser scanning microradiation of a tunable femtosecond-Ti:sapphire laser. Cellular response on autofluorescence to two-photon absorption of NIR radiation was compared with exposure to UVA light (one-photon absorption).

Paper Details

Date Published: 15 January 1996
PDF: 8 pages
Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); doi: 10.1117/12.229988
Show Author Affiliations
Karsten Koenig, Institute for Molecular Biotechnology (Germany)
Beckman Laser Institute and Medical Clinic (United States)
Peter T. C. So, Univ. of Illinois/Urbana-Champaign (United States)
William W. Mantulin, Univ. of Illinois/Urbana-Champaign (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)


Published in SPIE Proceedings Vol. 2628:
Optical and Imaging Techniques for Biomonitoring
Hans-Jochen Foth; Renato Marchesini; Halina Podbielska; Michel Robert-Nicoud; Herbert Schneckenburger, Editor(s)

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