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Proceedings Paper

Real-time fluorescence microscopy monitoring of porphyrin biodistribution
Author(s): Sol Kimel; Varda Gottfried; Karin Kunzi-Rapp; Nermin Akguen; Herbert Schneckenburger
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Paper Abstract

In vivo uptake of the natural porphyrins, uroporphyrin III (UP), coproporphyrin III (CP) and protoporphyrin IX (PP), was monitored by fluorescence microscopy. Experiments were performed using the chick chorioallantoic membrane (CAM) model, which allowed video documentation of fluorescence both in real time and after integration over a chosen time interval (usually 2 s). Sensitizers at a concentration of 50 (mu) M (100 (mu) L) were injected into a medium-sized vein (diameter approximately 40 micrometer) using an ultra-fine 10 micrometer diameter needle. Fluorescence images were quantitated by subtracting the fluorescence intensity of surrounding CAM tissue (Fmatrix) from the intravascular fluorescence intensity (Fintravascular), after transformation of the video frames into digital form. The differential fluorescence intensity, Fintravascular - Fmatrix, is a measure of the biodistribution. Real time measurements clearly showed that CP and UP fluorescence is associated with moving erythrocytes and not with endothelial cells of the vessel wall. Fluorescence intensity was monitored, up to 60 minutes after injection, by averaging the fluorescence over time intervals of 2 s and recording the integrated images. The fluorescence intensity reached its maximum in about 20 - 30 min after injection, presumably after monomerization inside erythrocyte membranes. The results are interpreted in terms of physical-chemical characteristics (e.g. hydrophilicity) and correlated with the photodynamically induced hemostasis in CAM blood vessels.

Paper Details

Date Published: 15 January 1996
PDF: 8 pages
Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); doi: 10.1117/12.229978
Show Author Affiliations
Sol Kimel, Technion-Israel Institute of Technology (Israel)
Varda Gottfried, Technion-Israel Institute of Technology (Israel)
Karin Kunzi-Rapp, Institut fur Lasertechnologien in der Medizin und Messtechnik, Univ. Ulm (Germany)
Nermin Akguen, Institut fur Lasertechnologien in der Medizin und Messtechnik, Univ. Ulm (Germany)
Herbert Schneckenburger, Fachhochschule Aalen and Institut fur Lasertechnologien in der Medizin und Messtechnik, Un (Germany)


Published in SPIE Proceedings Vol. 2628:
Optical and Imaging Techniques for Biomonitoring
Hans-Jochen Foth; Renato Marchesini; Halina Podbielska; Michel Robert-Nicoud; Herbert Schneckenburger, Editor(s)

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