Share Email Print
cover

Proceedings Paper • new

Macroscopic time-resolved imaging of tumor (Conference Presentation)

Paper Abstract

This presentation describes the development of the optical macroscanner and its application for metabolic imaging of large areas of tumors in mice. The scanner allows to interrogate areas as large as 15x15mm with the lateral resolution on the order of 15 micrometers. Acquisition times range from a few seconds for low pixel numbers to several minutes for high-resolution images. We present data for NAD(P)H imaging of tumor with genetically encoded mKate2. In addition, using macroscanner we demonstrated the possibility of visualizing caspase-3 activity using the FRET-biosensor TR23K, which is based on a pair of proteins - a red fluorescent protein as a donor and a chromoprotein as an acceptor. The in vivo assay was noninvasive and could be applied in strongly and weakly fluorescent subcutaneous xenografts in mice using the FLIM-FRET method.

Paper Details

Date Published: 14 March 2018
PDF
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104980V (14 March 2018); doi: 10.1117/12.2299317
Show Author Affiliations
Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany)
Marina Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)
Victoria Zherdeva, A.N. Bach Institute of Biochemistry (Russian Federation)
Alena Gavrina, Nizhny Novgorod State Medical Academy (Russian Federation)
Varvara Dudenkova, Nizhny Novgorod State Medical Academy (Russian Federation)
Elena Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)
Alexander Savitskiy, A.N. Bach Institute of Biochemistry (Russian Federation)
Wolfgang Becker, Becker & Hickl GmbH (Germany)


Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

© SPIE. Terms of Use
Back to Top