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Proceedings Paper

pEGFP transfection into murine skeletal muscle by electrosonoporation
Author(s): Mindaugas Tamošiūnas; Dainis Jakovels; Uldis Rubins; Roberts Kadikis; Ramona Petrovska; Saulius Šatkauskas
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Paper Abstract

In this study, we aimed to determine whether the combination of electroporation (EP) and ultrasound (US) waves (sonoporation) can affect the plasmid DNA transfection to mice tibialis cranialis muscle. Multispectral imaging technique combined with fluorescence spectroscopy point measurements has been used for the transcutaneous detection of enhanced green fluorescent protein (EGFP) fluorescence, providing information on location and duration of EGFP expression. We found that electrosonoporation, commonly enhancing pDNA transfection in vitro, had no positive effect on EGFP transfection efficiency increase in vivo with respect to electroporation alone. We presume that this may be associated with decreased viability of transfected fibers.

Paper Details

Date Published: 7 December 2017
PDF: 5 pages
Proc. SPIE 10592, Biophotonics—Riga 2017, 105920L (7 December 2017); doi: 10.1117/12.2297222
Show Author Affiliations
Mindaugas Tamošiūnas, Vytautas Magnus Univ. (Lithuania)
Dainis Jakovels, Univ. of Latvia (Latvia)
Uldis Rubins, Univ. of Latvia (Latvia)
Roberts Kadikis, Institute of Electronics and Computer Science (Latvia)
Ramona Petrovska, Latvian Biomedical Research and Study Ctr. (Latvia)
Saulius Šatkauskas, Vytautas Magnus Univ. (Lithuania)

Published in SPIE Proceedings Vol. 10592:
Biophotonics—Riga 2017
Janis Spigulis, Editor(s)

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