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Proceedings Paper

Implicit dosimetry of microorganism photodynamic inactivation
Author(s): Mindaugas Tamošiūnas; Neringa Kuliešienė; Rimantas Daugelavičius
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Paper Abstract

Photosensitization based antibacterial treatment is efficient against a broad range of pathogens but it utilizes suboptimal dosimetry with an explicit (and very broad range) determination of sensitizer concentration, light dose and fluence rates. In this study we verified the implicit dosimetry approach for pathogen photodynamic treatment, employing protoporphyrin IX (ppIX) photobleaching to assess the killing efficacy against Staphylococcus aureus and Candida albicans cells. The results show that there was an increased kill of S. aureus and C. albicans at higher degree of ppIX fluorescence decay. Therefore ppIX photobleaching can be incorporated into the PDI dose metric offering to predict the pathogen killing efficacy during photodynamic treatment.

Paper Details

Date Published: 7 December 2017
PDF: 4 pages
Proc. SPIE 10592, Biophotonics—Riga 2017, 105920K (7 December 2017); doi: 10.1117/12.2297221
Show Author Affiliations
Mindaugas Tamošiūnas, Vytautas Magnus Univ. (Lithuania)
Neringa Kuliešienė, Vytautas Magnus Univ. (Lithuania)
Rimantas Daugelavičius, Vytautas Magnus Univ. (Lithuania)

Published in SPIE Proceedings Vol. 10592:
Biophotonics—Riga 2017
Janis Spigulis, Editor(s)

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