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Proceedings Paper

Optimizing dynamic optical contrast imaging: signal characterization in the head and neck animal model (Conference Presentation)
Author(s): Karam W. Badran; Harrison Cheng; Shijun Sung; Peter Pellionisz; Zach Taylor; Warren S. Grundfest; Maie A. St John
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Paper Abstract

Objective: DOCI is a novel imaging modality with the ability to detect variations in endogenous fluorophore lifetimes by illuminating tissue with pulsed ultraviolet (UV) light. We have previously shown that DOCI is capable of delineating tumor margins. Tissue macro-/micro-environments, however, vary with organ site and histology. We therefore sought to better characterize DOCI signal analysis within the varying subsites of the oral cavity in this ex-vivo animal model. Design: Fresh ex-vivo oral cavity specimens (n=66) from three New Zealand white rabbits were harvested for pulsed UV illumination utilizing a 6-diode in-series DOCI system. Photons produced were detected and fluorophore lifetimes calculated over a specified, homogenous, region of interest. Specimen site, size, histology, and relative average DOCI values analyzed. Results: 66 specimens produced over 2 million data points for fluorophore lifetime analysis. The oral tongue muscle, dentition, and mucosa from the dorsal tongue, floor of mouth, and hard palate all produced unique DOCI relative average values. Each subsite was found to be uniquely different from one another and produced statistically significant differences in DOCI value (p<0.05). Conclusions: DOCI has the ability to distinguish subtle differences in oral cavity subsites following fresh ex vivo harvest. The fluorophore lifetime relative average values of each tissue is uniquely different posing a novel strategy for intra operative oncologic imaging, surveillance, and possibly aid in the workup of pre-cancerous lesions. Growing a repository of normal tissue subsites is crucial for integrating an automated real-time deep learning algorithm for rapid tissue analysis.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10469, Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2018, 1046914 (14 March 2018); doi: 10.1117/12.2296903
Show Author Affiliations
Karam W. Badran, UCLA David Geffen School of Medicine (United States)
Harrison Cheng, Univ. of California, Los Angeles (United States)
Shijun Sung, Univ. of California, Los Angeles (United States)
Peter Pellionisz, UCLA David Geffen School of Medicine (United States)
Zach Taylor, Univ. of California, Los Angeles (United States)
Warren S. Grundfest, Univ. of California, Los Angeles (United States)
Maie A. St John, UCLA David Geffen School of Medicine (United States)


Published in SPIE Proceedings Vol. 10469:
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2018
Brian J. F. Wong; Justus F. Ilgner; Max J. Witjes, Editor(s)

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