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Cryo-micro-optical sectioning tomography for label-free brainwide visualization of senile plaque (Conference Presentation)
Author(s): Yilin Luo; Anle Wang; Mengmeng Liu; Tian Lei; Xiaochuan Zhang; Zhaobing Gao; Hualiang Liang; Hui Gong; Jing Yuan
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Paper Abstract

Optical visualization of Alzheimer’s disease (AD) pathological changes is crucial to facilitate exploration of disease mechanism and treatment. We developed cryo-micro-optical sectioning tomography (cryo-MOST) to acquire brainwide map of senile plaques. Using intrinsic fluorescence emission intensified under ultra-low temperature, we accomplished senile plaque visualization at a micron-level resolution. A whole-brain coronal distribution of senile plaque in a transgenic mouse was successfully acquired without any exogenous dye. We believe cryo-MOST would be a potential tool for understanding neurodegenerative disease mechanism and evaluating drug efficacy.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104810M (14 March 2018); doi: 10.1117/12.2296253
Show Author Affiliations
Yilin Luo, Huazhong Univ. of Science and Technology (China)
Anle Wang, Wuhan National Lab. for Optoelectronics (China)
Huazhong Univ. of Science and Technology (China)
Mengmeng Liu, Wuhan National Lab. for Optoelectronics (China)
Huazhong Univ. of Science and Technology (China)
Tian Lei, Wuhan National Lab. for Optoelectronics (China)
Huazhong Univ. of Science and Technology (China)
Xiaochuan Zhang, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (China)
Zhaobing Gao, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (China)
Hualiang Liang, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (China)
Hui Gong, Wuhan National Lab. for Optoelectronics, Huazhong Univ. of Science and Technology (China)
Jing Yuan, Huazhong Univ. of Science and Technology, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10481:
Neural Imaging and Sensing 2018
Qingming Luo; Jun Ding, Editor(s)

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