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Proceedings Paper

Genotoxity of pyrolysis products originating from laser-treated porcine tissues
Author(s): Bernd Stocker; Ulla Plappert; Lothar W. Weber; Theodor M. Fliedner; Bernd Treffler; Thomas H. Meier
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Paper Abstract

Different types of porcine tissue were irradiated with a surgical CO2 laser. The generated aerosols were sampled on glass fiber filters and incubated with human peripheral blood cells. Afterwards, these exposed cells were subjected to the comet assay. The single cell gel electrophoresis or comet assay represents a powerful technique for the detection of DNA strand breaks in eukaryotic cells. In short, the electrophoretic mobility of DNA fragments is proportional to the quantity of DNA damage caused by the genotoxic agent in question. By investigating porcine tissue laser pyrolysis products, it is demonstrated that the comet assay is an appropriate tool to assess the genotoxic capacity of even a heterogeneously composed class of substances with unknown modes of action and interaction.

Paper Details

Date Published: 10 January 1996
PDF: 7 pages
Proc. SPIE 2624, Laser-Tissue Interaction and Tissue Optics, (10 January 1996); doi: 10.1117/12.229567
Show Author Affiliations
Bernd Stocker, Univ. Ulm (Germany)
Ulla Plappert, Univ. Ulm (Germany)
Lothar W. Weber, Univ. Ulm (Germany)
Theodor M. Fliedner, Univ. Ulm (Germany)
Bernd Treffler, Univ. Ulm (Germany)
Thomas H. Meier, Univ. Ulm (Germany)

Published in SPIE Proceedings Vol. 2624:
Laser-Tissue Interaction and Tissue Optics
Guy P. Delacretaz; Rudolf W. Steiner; Lars Othar Svaasand; Hansjoerg Albrecht; Thomas H. Meier, Editor(s)

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