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Proceedings Paper

Characterizing the hydration state of L-threonine in solution using terahertz time-domain attenuated total reflection spectroscopy
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Paper Abstract

The hydration of biomolecules is closely related to the dynamic process of their functional expression, therefore, characterizing hydration phenomena is a subject of keen interest. However, direct measurements on the global hydration state of biomolecules couldn’t have been acquired using traditional techniques such as thermodynamics, ultrasound, microwave spectroscopy or viscosity, etc. In order to realize global hydration characterization of amino acid such as L-threonine, terahertz time-domain attenuated total reflectance spectroscopy (THz-TDS-ATR) was adopted in this paper. By measuring the complex permittivity of L-threonine solutions with various concentrations in the THz region, the hydration state and its concentration dependence were obtained, indicating that the number of hydrous water decreased with the increase of concentration. The hydration number was evaluated to be 17.8 when the molar concentration of L-threonine was 0.34 mol/L, and dropped to 13.2 when the molar concentration increased to 0.84 mol/L, when global hydration was taken into account. According to the proposed direct measurements, it is believed that the THz-TDS-ATR technique is a powerful tool for studying the picosecond molecular dynamics of amino acid solutions.

Paper Details

Date Published: 12 January 2018
PDF: 8 pages
Proc. SPIE 10623, 2017 International Conference on Optical Instruments and Technology: IRMMW-THz Technologies and Applications, 106230N (12 January 2018); doi: 10.1117/12.2295512
Show Author Affiliations
Huachuan Huang, China Academy of Engineering Physics (China)
Nanjing Univ. of Science and Technology (China)
Southwest Univ. of Science and Technology (China)
Qiao Liu, China Academy of Engineering Physics (China)
Liguo Zhu, China Academy of Engineering Physics (China)
Zeren Li, China Academy of Engineering Physics (China)


Published in SPIE Proceedings Vol. 10623:
2017 International Conference on Optical Instruments and Technology: IRMMW-THz Technologies and Applications
Cunlin Zhang; Xi-Cheng Zhang; Zhiming Huang; Liquan Dong, Editor(s)

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