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Proceedings Paper

Using luminescent ruthenuim(II) complexes as dyes in the multiplex concept
Author(s): Brigitte Ullrich; Stefan Seeger; Juergen M. Wolfrum
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Paper Abstract

The detection of several substances in a mixture can be realized by using luminescent markers and discriminating them by their luminescence lifetimes. The number of simultaneously detectable analytes can be increased by extending the time region of the lifetimes of the dyes. The fluorescence lifetimes of commercial available organic dyes do not exceed 100 ns. Longer decays are found among the complexes of transition metals. Therefore the photophysical properties of a number of luminescent ruthenium(II) tris(alpha-diimine) complexes in air equilibrated aqueous buffer solution were measured at room temperature. Their application as luminescence markers in diagnostics and analytics is discussed. The possibility of emission enhancement by addition of detergents to reduce water quenching and by addition of sodiumsulfite to reduce oxygen quenching was investigated. Some of the ruthenium compounds were encapsulated in carboxylated polystyrol beads. Resulting changes of the luminescence decays are presented. The possibility of increasing sensitivity by multilabeling with dyed beads in analytical applications is discussed.

Paper Details

Date Published: 8 January 1996
PDF: 8 pages
Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229516
Show Author Affiliations
Brigitte Ullrich, Ruprecht-Karls-Univ. Heidelberg (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Germany)
Juergen M. Wolfrum, Ruprecht-Karls-Univ. Heidelberg (Germany)

Published in SPIE Proceedings Vol. 2629:
Biomedical Optoelectronics in Clinical Chemistry and Biotechnology
Stefan Andersson-Engels; Mario Corti; Ivan Kertesz; Norbert Kroo; Heinz P. Weber; Terence A. King; Riccardo Pratesi; Stefan Seeger, Editor(s)

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