Share Email Print
cover

Proceedings Paper • new

Metabolic imaging of tumor for diagnosis and response for therapy
Author(s): Elena Zagaynova; Marina Shirmanova; Maria Lukina; Varvara Dudenkova; Nadezgda Ignatova; Vadim Elagin; Irena Shlivko; Vladislav Scheslavsky; Natalia Orlinskay
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Nonlinear optical microscopy combined with fluorescence lifetime imaging is a non-invasive imaging technique, based on the study of fluorescence decay times of naturally occurring fluorescent molecules, enabling a noninvasive investigation of the biological tissue with subcellular resolution. Cancer exhibits altered cellular metabolism, which affects the autofluorescence of metabolic cofactors NAD(P)H and FAD. In this study features of tumor metabolism in different systems of organization (from cell culture to patient lesion) was showed. The observed differences in the relative contributions of free NAD(P)H and FAD testify to an increased a glycolytic metabolism in cancer cells compare to fibroblasts. In 3D spheroids, the cells of the proliferating zone had greater a1 and lower tm values than the cells of the quiescent zone, which likely is a consequence of their higher glycolytic rate. During the growth of colorectal cancer in the experimental mouse model, the contribution of the free component of NAD(P)H was increased. Dysplastic nevus and melanoma is characterized by raised contribution of free NADH compare to healthy skin. Therefore, melanoma cells had very short value of τ1.

Paper Details

Date Published: 23 February 2018
PDF: 8 pages
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049804 (23 February 2018); doi: 10.1117/12.2295127
Show Author Affiliations
Elena Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)
Marina Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)
Maria Lukina, Nizhny Novgorod State Medical Academy (Russian Federation)
Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
Varvara Dudenkova, Nizhny Novgorod State Medical Academy (Russian Federation)
Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation)
Nadezgda Ignatova, Nizhny Novgorod State Medical Academy (Russian Federation)
Vadim Elagin, Nizhny Novgorod State Medical Academy (Russian Federation)
Irena Shlivko, Nizhny Novgorod Research Institute of Traumatology and Orthopedics (Russian Federation)
Vladislav Scheslavsky, Becker & Hickl GmbH (Germany)
Natalia Orlinskay, Nizhny Novgorod Research Institute of Traumatology and Orthopedics (Russian Federation)


Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

© SPIE. Terms of Use
Back to Top