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Proceedings Paper

Immobilized fluorescent dyes for sensitive pH measurements on enamel surfaces with fiber optics
Author(s): A. Rumphorst; Stefan Seeger; H. Duschner
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Paper Abstract

Information on the pH directly on surfaces of dental enamel is an important aspect in research on tooth decay. As an alternative to pH-electrodes our approach to the problem is the optical determination of pH by pH sensitive fluorescent dyes immobilized to tooth surfaces. In this study a model for measuring pH either on aminated cellulose substrates or on enamel (in vitro) with a fluorescein type dye is presented. The experimental realization is a fiber optic sensor with a nitrogen-pumped dye laser system and photodiode for the detection of the emitted fluorescence light. The surface pH values in the range between 4 and 7 were derived from the ratios of the excitation bands at 490 nm and 460 nm.

Paper Details

Date Published: 8 January 1996
PDF: 10 pages
Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229508
Show Author Affiliations
A. Rumphorst, Ruprecht-Karls-Univ. Heidelberg (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Germany)
H. Duschner, Universitaetsklinik Mainz (Germany)

Published in SPIE Proceedings Vol. 2629:
Biomedical Optoelectronics in Clinical Chemistry and Biotechnology
Stefan Andersson-Engels; Mario Corti; Ivan Kertesz; Norbert Kroo; Heinz P. Weber; Terence A. King; Riccardo Pratesi; Stefan Seeger, Editor(s)

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