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Proceedings Paper

Cellulose antibody films for highly specific evanescent wave immunosensors
Author(s): Andreas Hartmann; Daniel Bock; Thomas Jaworek; Sepp Kaul; Matthais Schulze; H. Tebbe; Gerhard Wegner; Stefan Seeger
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Paper Abstract

For the production of recognition elements for evanescent wave immunosensors optical waveguides have to be coated with ultrathin stable antibody films. In the present work non amphiphilic alkylated cellulose and copolyglutamate films are tested as monolayer matrices for the antibody immobilization using the Langmuir-Blodgett technique. These films are transferred onto optical waveguides and serve as excellent matrices for the immobilization of antibodies in high density and specificity. In addition to the multi-step immobilization of immunoglobulin G(IgG) on photochemically crosslinked and oxidized polymer films, the direct one-step transfer of mixed antibody-polymer films is performed. Both planar waveguides and optical fibers are suitable substrates for the immobilization. The activity and specificity of immobilized antibodies is controlled by the enzyme-linked immunosorbent assay (ELISA) technique. As a result reduced non-specific interactions between antigens and the substrate surface are observed if cinnamoylbutyether-cellulose is used as the film matrix for the antibody immobilization. Using the evanescent wave senor (EWS) technology immunosensor assays are performed in order to determine both the non-specific adsorption of different coated polymethylmethacrylat (PMMA) fibers and the long-term stability of the antibody films. Specificities of one-step transferred IgG-cellulose films are drastically enhanced compared to IgG-copolyglutamate films. Cellulose IgG films are used in enzymatic sandwich assays using mucine as a clinical relevant antigen that is recognized by the antibodies BM2 and BM7. A mucine calibration measurement is recorded. So far the observed detection limit for mucine is about 8 ng/ml.

Paper Details

Date Published: 8 January 1996
PDF: 11 pages
Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229507
Show Author Affiliations
Andreas Hartmann, Ruprecht-Karls-Univ. Heidelberg (Germany)
Daniel Bock, Ruprecht-Karls-Univ. Heidelberg (Germany)
Thomas Jaworek, Max-Planck-Institut fuer Polymerforschung (Germany)
Sepp Kaul, Universitaetsfrauenklinik Heidelberg (Germany)
Matthais Schulze, Max-Planck-Institut fuer Polymerforschung (Germany)
H. Tebbe, Max-Planck-Institut fuer Polymerforschung (Germany)
Gerhard Wegner, Max-Planck-Institut fuer Polymerforschung (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Switzerland)

Published in SPIE Proceedings Vol. 2629:
Biomedical Optoelectronics in Clinical Chemistry and Biotechnology
Stefan Andersson-Engels; Mario Corti; Ivan Kertesz; Norbert Kroo; Heinz P. Weber; Terence A. King; Riccardo Pratesi; Stefan Seeger, Editor(s)

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