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Proceedings Paper

Cell patterns for biosensors manufactured with laser tweezers
Author(s): Joachim Renken; Stefan Seeger
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Paper Abstract

A drug screening assay based on patterned cells was developed. The patterning was performed by detaching single lymphocytes with laser tweezers from a poly-ethylene oxide (PEO) surface and immobilizing them on a surface coated with Cell-TakR, a strong cellular adhesive. The detachment force of the cells from the PEO-surface was determined on a single cell base with the laser tweezers to be between 1.5 and 4.5 pN. Different lymphocyte classes were loaded with the fluorescent calcium indicator Fluo-3 and the pH indicator BCECF. Specific stimulation of the immobilized cells is monitored.

Paper Details

Date Published: 8 January 1996
PDF: 9 pages
Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229505
Show Author Affiliations
Joachim Renken, Ruprecht-Karls-Univ. Heidelberg (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Germany)


Published in SPIE Proceedings Vol. 2629:
Biomedical Optoelectronics in Clinical Chemistry and Biotechnology
Stefan Andersson-Engels; Mario Corti; Ivan Kertesz; Norbert Kroo; Heinz P. Weber; Terence A. King; Riccardo Pratesi; Stefan Seeger, Editor(s)

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