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Proceedings Paper

Compact microscope-based 850-nm optical tweezers
Author(s): Carlo Frediani; S. Grego; L. Guidoni; Ennio Arimondo
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Paper Abstract

Emission from a single mode 100 mW laser diode at 850 nm is used for realizing optical tweezers: the laser beam is introduced into a microscope and focused by the objective into the object plane. Injection of the beam into a 40X microscope objective has been studied and the position and the size of the waist measured. The trap performance was studied as a function of the dimensions of the trapped particles. Trapping of polystyrene latex spheres of different size (from 0.2 micrometer to 6 micrometer) was observed in different conditions of laser power and transverse velocity of the spheres. Biological objects, Tetraselmis, of large dimension (around 10 micrometer) were also studied. We demonstrate the existence of an optimal range of size of the particles to be trapped. Furthermore we measure minimum trapping power required for trapping and the maximum speed of the trapped objects as a function of the dimensions.

Paper Details

Date Published: 8 January 1996
PDF: 6 pages
Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229502
Show Author Affiliations
Carlo Frediani, Istituto di Biofisica del CNR (Italy)
S. Grego, Univ. di Pisa and GNSM/CNR (Italy)
L. Guidoni, Univ. di Pisa (Italy)
Ennio Arimondo, Univ. di Pisa (Italy)

Published in SPIE Proceedings Vol. 2629:
Biomedical Optoelectronics in Clinical Chemistry and Biotechnology
Stefan Andersson-Engels; Mario Corti; Ivan Kertesz; Norbert Kroo; Heinz P. Weber; Terence A. King; Riccardo Pratesi; Stefan Seeger, Editor(s)

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