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Interrogation of evolving tumor vasculature using high-resolution CT imaging and a nanoparticle contrast agent
Author(s): Ketan B. Ghaghada; Zbigniew Starosolski; Igor Stupin; Poonam Sarkar; Ananth Annapragada
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Paper Abstract

The architecture of intra-tumoral vascular network continuously evolves with tumor progression. Non-invasive methods that facilitate 3D in vivo interrogation of tumor vascular architecture could improve understanding of tumor progression and metastasis. In this work, we studied evolving tumor vasculature using high-resolution CT images and a blood-pool, nanoparticle iodinated contrast agent. In vivo studies were performed in a transgenic mouse model of neuroblastoma that exhibit spontaneous bilateral tumors in the adrenals. Animals were divided into three groups based on tumor age: early-age tumor, intermediate-age tumor, old-age tumor. Tumor progression was monitored using T2-weighted MRI. Contrast-enhanced CT imaging was performed at two points: the first imaging session (leak map) was performed 4 days after administration of the nanoparticle agent to interrogate changes in tumor vascular permeability. Immediately thereafter, a second dose of contrast agent was administered and CT imaging was performed within 1 hour to capture high-resolution angiograms of tumor vasculature. CT angiograms demonstrated the highly-vascularized nature of these tumors. Old-age tumors exhibited a higher fractional volume of avascular regions and an increased number of large superficial blood vessels on tumor periphery. Old-age tumors also demonstrated the presence of intra-vessel tumor thrombus and the invasion of tumor into the inferior vena cava. Leak maps images demonstrated signal enhancement throughout the tumor in early-age tumors, including the core region, suggestive of the presence of highly permeable blood vessels through the tumor volume. Old-age tumors exhibited relatively lower signal enhancement, indicative of a less 'leaky' tumor vascular network compared to early and intermediate-stage tumors.

Paper Details

Date Published: 12 March 2018
PDF: 7 pages
Proc. SPIE 10578, Medical Imaging 2018: Biomedical Applications in Molecular, Structural, and Functional Imaging, 105781C (12 March 2018); doi: 10.1117/12.2293607
Show Author Affiliations
Ketan B. Ghaghada, Texas Children's Hospital (United States)
Zbigniew Starosolski, Texas Children's Hospital (United States)
Igor Stupin, Texas Children's Hospital (United States)
Poonam Sarkar, Baylor College of Medicine (United States)
Ananth Annapragada, Texas Children's Hospital (United States)


Published in SPIE Proceedings Vol. 10578:
Medical Imaging 2018: Biomedical Applications in Molecular, Structural, and Functional Imaging
Barjor Gimi; Andrzej Krol, Editor(s)

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