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Proceedings Paper

3D imaging of theranostic nanoparticles in mice organs by means of x-ray phase contrast tomography
Author(s): E. Longo; A. Bravin; F. Brun; I. Bukreeva; A. Cedola; O. De La Rochefoucauld; M. Fratini; X. Le Guevel; L. Massimi; L. Sancey; O. Tillement; P. Zeitoun
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Paper Abstract

Theranostics is an innovative research field that aims to develop high target specificity cancer treatments by administering small metal-based nanoparticles (NPs). This new generation of compounds exhibits diagnostic and therapeutic properties due to the high atomic number of their metal component. In the framework of a combined research program on low dose X-ray imaging and theranostic NPs, X-ray Phase Contrast Tomography (XPCT) was performed at ESRF using a 3 μm pixel optical system on two samples: a mouse brain bearing melanoma metastases injected with gadolinium NPs and, a mouse liver injected with gold NPs. XPCT is a non-destructive technique suitable to achieve the 3D reconstruction of a specimen and, widely used at micro-scale to detect abnormalities of the vessels, which are associated to the tumor growth or to the development of neurodegenerative diseases. Moreover, XPCT represents a promising and complementary tool to study the biodistribution of theranostic NPs in biological materials, thanks to the strong contrast with respect to soft tissues that metal-based NPs provide in radiological images. This work is relied on an original imaging approach based on the evaluation of the contrast differences between the images acquired below and above K-edge energies, as a proof of the certain localization of NPs. We will present different methods aiming to enhance the localization of NPs and a 3D map of their distribution in large volume of tissues.

Paper Details

Date Published: 9 March 2018
PDF: 7 pages
Proc. SPIE 10573, Medical Imaging 2018: Physics of Medical Imaging, 105734I (9 March 2018); doi: 10.1117/12.2293090
Show Author Affiliations
E. Longo, Lab. d’Optique Appliquée, ENSTA-CNRS-Ecole Polytechnique-Univ. Paris-Saclay (France)
A. Bravin, European Synchrotron Radiation Facility (France)
F. Brun, Institute of Nanotechnology-CNR (Italy)
I. Bukreeva, Institute of Nanotechnology-CNR (Italy)
A. Cedola, Institute of Nanotechnology-CNR (Italy)
O. De La Rochefoucauld, Imagine Optic SA (France)
M. Fratini, Institute of Nanotechnology-CNR (Italy)
IRCCS Santa Lucia Foundation, MARBLab (Italy)
X. Le Guevel, Institute for Advanced Biosciences, CNRS, Univ. Grenoble Alpes (France)
L. Massimi, Institute of Nanotechnology-CNR (Italy)
L. Sancey, Institute for Advanced Biosciences, CNRS, Univ. Grenoble Alpes (France)
O. Tillement, Institut Lumière-Matière, Univ. Claude Bernard Lyon1-CNRS, Univ. de Lyon (France)
P. Zeitoun, Lab. d’Optique Appliquée, ENSTA-CNRS-Ecole Polytechnique-Univ. Paris-Saclay (France)

Published in SPIE Proceedings Vol. 10573:
Medical Imaging 2018: Physics of Medical Imaging
Joseph Y. Lo; Taly Gilat Schmidt; Guang-Hong Chen, Editor(s)

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