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Proceedings Paper

An add-on adaptive optical module for laser scanning microscopy (Conference Presentation)
Author(s): Ichun Anderson Chen; Wenzhi Sun; Yajie Liang; Daniel Milkie; Thomas Bifano; Na Ji

Paper Abstract

Wavefront-shaping devices incorporated into optical microscopy systems are capable of correcting sample-induced aberrations and recovering diffraction-limited imaging performance. The widespread dissemination and application of adaptive optical techniques, however, requires easy integration of adaptive optical modules, both in terms of hardware and software, into existing microscopes. We built an adaptive optical module with reduced complexity and simplified integration by utilizing a novel segmented deformable mirror and a standalone control software program. We demonstrated its ability to improve image brightness and resolution at depth in the mouse, zebrafish, and fly brains in vivo.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105020F (14 March 2018); doi: 10.1117/12.2292944
Show Author Affiliations
Ichun Anderson Chen, Howard Hughes Medical Institute (United States)
Wenzhi Sun, Howard Hughes Medical Institute (United States)
Yajie Liang, Howard Hughes Medical Institute (United States)
Daniel Milkie, Howard Hughes Medical Institute (United States)
Thomas Bifano, Boston Univ. (United States)
Na Ji, Howard Hughes Medical Institute (United States)


Published in SPIE Proceedings Vol. 10502:
Adaptive Optics and Wavefront Control for Biological Systems IV
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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