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Proceedings Paper

Low-cost polarization microscopy for cholesterol crystals
Author(s): Kyungmin Kim; Seonghee Cho; Taehoon Kim; Hyoeun Park; Jinmoo Kim; Seunghoon Lee; Yeonsu Kang; Kiyuk Chang; Chulhong Kim
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Paper Abstract

Because cholesterol crystals (Chcs) are a major cause of atherosclerosis, imaging Chcs in tissues with high sensitivity and specificity is important in diagnosing and predicting atherosclerosis. Polarizing microscopy (PM) has been widely used to image crystalline materials in tissues, but it has been difficult to distinguish Chcs from other crystalline materials in tissues. Thus, various methods such as fluorescent dye staining, Raman spectroscopy, and two-photon microscopy (TPM) have been developed to image Chcs with high sensitivity and specificity. However, these methods require expensive equipment or complex processes. Therefore, we have developed a low-cost, easy-to-use PM system using an LED light source that can distinguish Chcs from other crystalline materials with high sensitivity and specificity. Due to the nature of the LED spectrum in our system, collagen is displayed in yellow and Chcs in blue. In addition, we have improved the sensitivity and specificity by creating an aqueous condition on the sample. In the aqueous state, signals of yellowish collagen fibers were reduced and signals of Chcs were highlighted. The Chcs detection capability of our system was verified compared with the TPM image. In addition, clinical feasibility was shown by comparison with existing histological methods.

Paper Details

Date Published: 20 February 2018
PDF: 5 pages
Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104971O (20 February 2018); doi: 10.1117/12.2292855
Show Author Affiliations
Kyungmin Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
Seonghee Cho, Pohang Univ. of Science and Technology (Korea, Republic of)
Taehoon Kim, The Catholic Univ. of Korea (Korea, Republic of)
Hyoeun Park, The Catholic Univ. of Korea (Korea, Republic of)
Jinmoo Kim, The Catholic Univ. of Korea (Korea, Republic of)
Seunghoon Lee, The Catholic Univ. of Korea (Korea, Republic of)
Yeonsu Kang, The Catholic Univ. of Korea (Korea, Republic of)
Kiyuk Chang, The Catholic Univ. of Korea (Korea, Republic of)
Chulhong Kim, Pohang Univ. of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 10497:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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