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Proceedings Paper

Conjugate adaptive optics with remote focusing in multiphoton microscopy
Author(s): Xiaodong Tao; Tuwin Lam; Bingzhao Zhu; Qinggele Li; Marc R. Reinig; Joel Kubby
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Paper Abstract

The small correction volume for conventional wavefront shaping methods limits their application in biological imaging through scattering media. In this paper, we take advantage of conjugate adaptive optics (CAO) and remote focusing (CAORF) to achieve three-dimensional (3D) scanning through a scattering layer with a single correction. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We demonstrate two-photon imaging with CAORF through mouse skull. The fluorescent microspheres embedded under the scattering layers can be clearly observed after applying the correction.

Paper Details

Date Published: 23 February 2018
PDF: 6 pages
Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105020E (23 February 2018); doi: 10.1117/12.2292492
Show Author Affiliations
Xiaodong Tao, Univ. of California, Santa Cruz (United States)
Tuwin Lam, Univ. of California, Santa Cruz (United States)
Bingzhao Zhu, Univ. of California, Santa Cruz (United States)
Qinggele Li, Univ. of California, Santa Cruz (United States)
Marc R. Reinig, Univ. of California, Santa Cruz (United States)
Joel Kubby, Univ. of California, Santa Cruz (United States)


Published in SPIE Proceedings Vol. 10502:
Adaptive Optics and Wavefront Control for Biological Systems IV
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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