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Proceedings Paper

3D fluorescence microscopy with multi-shot coded aperture measurements (Conference Presentation)

Paper Abstract

Coded aperture imaging for 3D reconstructions have been applied in both photography and microscopy to successfully recover depth information from images captured with a patterned pupil plane. Generally, single-shot coded aperture microscopy methods require the 3D sample to be extremely sparse and with no overlapping features. Here, we extend coded aperture microscopy methods to multi-shot 3D fluorescent imaging with wave-optical forward models, enabling thicker and denser 3D samples to be faithfully reconstructed with efficient data capture via compressed sensing and advanced inverse algorithms.. We demonstrate successful reconstruction of a 500um thick brine shrimp sample. We further study the minimum required number of codes to capture full fluorescent information under wide-field illumination The experiments are carried out with a 4f system and a spatial light modulator in the Fourier (aperture) domain to display the codes.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991B (14 March 2018); doi: 10.1117/12.2292478
Show Author Affiliations
Hsiou-Yuan Liu, Univ. of California, Berkeley (United States)
Laura Waller, Univ. of California, Berkeley (United States)


Published in SPIE Proceedings Vol. 10499:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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