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PDI using nebulized indocyanine green for pneumonia treatment
Author(s): Mariana C. Geralde; Giulia Kassab; Natalia M. Inada; Cristina Kurachi; Vanderlei S. Bagnato
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Paper Abstract

Infectious pneumonia is a major cause of morbidity/mortality, mainly due to the increasing rate of microorganisms resistant to antibiotics. Photodynamic Inactivation (PDI) is emerging as a promising treatment option, which effects are based on oxidative stress, targeting several biomolecules and probably preventing potential resistant strains. In previous studies, the in vitro inactivation of Streptococcus pneumoniae using indocyanine green (ICG) and infrared (IR) light source (780 nm) was successful, and achieving satisfactory reduction of colony-forming units (CFU/mL). In the present study, a proof-of-principle protocol was designed to treat lung infections by PDI using extracorporeal irradiation with a 780 nm laser device and nebulized ICG as photosensitizer. Balb/c mice were infected with S. pneumoniae and PDI was performed two days after infection using 800 μM of nebulized ICG and extracorporeal irradiation. Our results indicate that IR-extracorporeal PDI using nebulized ICG may be considered a potential pneumonia treatment, and pulmonary decontamination with PDI may be used as a single therapy or as an adjuvant for antibiotics.

Paper Details

Date Published: 14 February 2018
PDF: 6 pages
Proc. SPIE 10470, Endoscopic Microscopy XIII, 104700H (14 February 2018); doi: 10.1117/12.2291987
Show Author Affiliations
Mariana C. Geralde, Univ. of São Paulo (Brazil)
Giulia Kassab, Univ. of São Paulo (Brazil)
Natalia M. Inada, Univ. of São Paulo (Brazil)
Cristina Kurachi, Univ. of São Paulo (Brazil)
Vanderlei S. Bagnato, Univ. of São Paulo (Brazil)


Published in SPIE Proceedings Vol. 10470:
Endoscopic Microscopy XIII
Guillermo J. Tearney; Thomas D. Wang; Melissa J. Suter, Editor(s)

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