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Handheld dual-modality wide-field fluorescent imaging guided dual-axis confocal microscope for fluorescence molecular guidance of precise tumor resection in head and neck surgery (Conference Presentation)
Author(s): Zhen Qiu; Tarn Teraphongphom; Nynke S. van den Berg; Nathan O. Loewke; Guolan Lu; Robert Ertsey; Frank Schonig; Stephan Rogalla; Shai Friedland; Michael J. Mandella; Eben L. Rosenthal; Christopher H. Contag
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Paper Abstract

Wide-field fluorescent imaging for fluorescence molecular guidance has become a promising technique for use in imaging guided surgical navigation, but quick and intuitive microscopic inspection of fluorescent hot spots is still needed to confirm local disease states of tissues. To address this unmet need, we have developed a clinically translatable dual-modality handheld surgical microscope that incorporates both, wide-field (mesoscopic) fluorescence imaging and high-resolution (microscopic) horizontal optical-sectioning. This is accomplished by integrating a commercially available wide-field fiberscope, modified for two-color (660nm and 785nm) fluorescent detection, into a compact package (5.5 mm dia.) which also contains a dual-axis confocal (DAC) microscope. DAC microscopy is a high-sensitivity, high-resolution fluorescent imaging technology that benefits from the specificity of molecular probes, and enables interrogation of deeper regions of tissue by performing optical-sectioning of tissue. The DAC microscope has been designed with custom catadioptric micro-lenses to provide broadband multispectral capability for fluorescence imaging of multiple fluorophores over a broad spectral range (VIS to NIR), and also uses a novel MEMS-based scanning system for horizontal sectioning, and thus enables access to deeper regions of tissue at resolutions comparable to histological analysis. Large field-of-view (mm scale) is further provided by image mosaicing. The instrument thus provides simultaneous mesoscopic and microscopic fluorescence imaging over a broad spectral range for intuitively performing fast in-vivo search and microscopic confirmation of optical molecular markers in tissue, which is a capability that will become increasingly important for precise tumor resection in oncology as more optical molecular markers become approved for human use.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10478, Molecular-Guided Surgery: Molecules, Devices, and Applications IV, 104780E (14 March 2018); doi: 10.1117/12.2291986
Show Author Affiliations
Zhen Qiu, Institute of Quantitative Health Science and Engineering, Michigan State Univ. (United States)
Tarn Teraphongphom, Stanford Univ. (United States)
Nynke S. van den Berg, Stanford Univ. (United States)
Nathan O. Loewke, Stanford Univ. (United States)
Guolan Lu, Stanford Univ. (United States)
Robert Ertsey, Stanford Univ. (United States)
Frank Schonig, Stanford Univ. (United States)
Stephan Rogalla, Stanford Univ. (United States)
Shai Friedland, Stanford Univ. (United States)
Michael J. Mandella, Stanford Univ. (United States)
Eben L. Rosenthal, Stanford Univ. (United States)
Christopher H. Contag, Institute of Quantitative Health Science and Engineering, Michigan State Univ. (United States)


Published in SPIE Proceedings Vol. 10478:
Molecular-Guided Surgery: Molecules, Devices, and Applications IV
Brian W. Pogue; Sylvain Gioux; Greg Biggs, Editor(s)

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