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Proceedings Paper

Room temperature phosphorescent sensing probe for detection of DNA
Author(s): Marta Elena Diaz-Garcia; M. Roza-Fernandez
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Paper Abstract

The interaction between double-stranded DNA and a luminescent Pd-porphyrin complex has been studied by room temperature phosphorescence (RTP). Intercalation of the Pd complex into DNA in deoxygenated solution is accompanied by an observed enhanced RTP emission centered at 680 nm. Analysis of the RTP spectral data gave a value of 1.0 multiplied by 106 M-1 for the Pd complex-DNA association constant and the complex seemed to bind DNA at the GC rich environments. The spectroscopic features of this interaction and the analytical performance characteristics of the RTP method for DNA determination are evaluated. The implication of the use of the Pd-complex RTP probe in combination with time resolved luminescence measurements are discussed.

Paper Details

Date Published: 3 January 1995
PDF: 8 pages
Proc. SPIE 2631, Medical and Fiber Optic Sensors and Delivery Systems, (3 January 1995); doi: 10.1117/12.229181
Show Author Affiliations
Marta Elena Diaz-Garcia, Univ. de Oviedo (Spain)
M. Roza-Fernandez, Univ. de Oviedo (Spain)

Published in SPIE Proceedings Vol. 2631:
Medical and Fiber Optic Sensors and Delivery Systems
Nathan I. Croitoru; Mitsunobu Miyagi; Guillermo Orellana; Annamaria Verga Scheggi; Henricus J. C. M. Sterenborg, Editor(s)

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