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Antimicrobial blue light inactivation of Neisseria gonorrhoeae
Author(s): Ying Wang; Ying Gu; Tianhong Dai
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Paper Abstract

Neisseria gonorrhoeae is a human-adapted, gram-negative diplococcus that infects human reproductive tracts and causes gonorrhea, a sexually transmitted disease, resulting in discharge and inflammation at the urethra, cervix, pharynx, or rectum. Over the years, N. gonorrhoeae has developed resistance to nearly every drug ever used to treat it, including sulfonamides, penicillin, tetracycline, and fluoroquinolones. Drug-resistant N. gonorrhoeae is now considered by the Centers for Disease Control and Prevention (CDC) as an urgent threat. The present study aimed to evaluate the efficacy of antimicrobial blue light (aBL) at 405 and 470 nm for inactivating N. gonorrhoeae and reveal the mechanism of action. Our results showed that an exposure of 45 J/cm2 aBL at 405 nm reduced the bacterial CFU by 7.16-log10. When the aBL exposure was increased to 54 J/cm2, eradication of bacterial CFU was achieved. When the bacteria were exposed to aBL at 470 nm, 3-log10 reduction of CFU was observed at an aBL exposure of higher than 126 J/cm2. Absorption and fluorescence spectroscopic analyses revealed the presence of endogenous porphyrins and flavins in N. gonorrhoeae cells. The present study indicated that aBL is a potential strategy to control N. gonorrhoeae infections. Endogenous porphyrins play a vital role in the killing effects of aBL. In vivo experiments are ongoing in our laboratory to treat genital tract infections in mice using aBL and explore the potential clinical applications.

Paper Details

Date Published: 8 February 2018
PDF: 6 pages
Proc. SPIE 10479, Light-Based Diagnosis and Treatment of Infectious Diseases, 104790O (8 February 2018); doi: 10.1117/12.2291545
Show Author Affiliations
Ying Wang, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Chinese PLA General Hospital (China)
Ying Gu, Chinese PLA General Hospital (China)
Tianhong Dai, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 10479:
Light-Based Diagnosis and Treatment of Infectious Diseases
Tianhong Dai, Editor(s)

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