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Proceedings Paper

Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields
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Paper Abstract

The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.

Paper Details

Date Published: 20 February 2018
PDF: 5 pages
Proc. SPIE 10506, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV, 1050610 (20 February 2018); doi: 10.1117/12.2291449
Show Author Affiliations
Kiheung Kim, Yonsei Univ. (Korea, Republic of)
Wonju Lee, Yonsei Univ. (Korea, Republic of)
Kyungwha Chung, Ewha Womans Univ. (Korea, Republic of)
Hongki Lee, Yonsei Univ. (Korea, Republic of)
Taehwang Son, Yonsei Univ. (Korea, Republic of)
Youngjin Oh, Samsung Display Co., Ltd. (Korea, Republic of)
Yun-Feng Xiao, Peking Univ. (China)
Dong Ha Kim, Ewha Womans Univ. (Korea, Republic of)
Donghyun Kim, Yonsei Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 10506:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV
Alexander N. Cartwright; Dan V. Nicolau; Dror Fixler, Editor(s)

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