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Proceedings Paper

Raman spectroscopic analysis for gastric and colorectal cancer in surgical treatment toward molecular-guided surgery
Author(s): Shigehiro Koga; Yuji Watanabe; Yusuke Oshima
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Paper Abstract

Raman spectroscopy provides a wealth of diagnostic information to the surgeon with in situ cancer detection and label-free histopathology in intraoperative conditions. Raman spectroscopy is a promising optical technique which can analyze biological tissues with light scattering. The difference in frequencies between the incident light and the scattering light are called Raman shifts, which correspond to the vibrational energy of the molecular bonds. Raman spectrum gives information about the molecular structure and composition in biological specimens. We had been previously reported that Raman spectroscopy could distinguish various histological types of human lung cancer cells from normal cells in vitro, and also confirmed that Raman spectra obtained from cancer cells and their environment including other cells and extracellular matrix in xenograft models and spontaneous metastasis models were distinguishable using Raman spectroscopy combined with fluorescence microscopy and photoluminescence imaging. Malignancy can be characterized not only by the cancer cells but also by the environmental factors including immune cells, stroma cells, secretion vesicles and extracellular matrix, but to identify and detect cancer diagnostic biomarkers in vivo on Raman spectroscopy is still challenging. Here we investigate morphological and molecular dynamics in advanced cancer specimens obtained from patients. We are also constructing a customdesigned Raman spectral imaging system for both in vitro and in vivo assay of tumor tissues to reveal the metastasis process and to evaluate therapeutic effects of anti-cancer drugs and their drug delivery toward the clinical application of the technique.

Paper Details

Date Published: 22 February 2018
PDF: 5 pages
Proc. SPIE 10478, Molecular-Guided Surgery: Molecules, Devices, and Applications IV, 1047806 (22 February 2018); doi: 10.1117/12.2291435
Show Author Affiliations
Shigehiro Koga, Ehime Univ. Graduate School of Medicine (Japan)
Yuji Watanabe, Ehime Univ. Graduate School of Medicine (Japan)
Yusuke Oshima, Ehime Univ. Hospital (Japan)
Tohoku Univ. (Japan)

Published in SPIE Proceedings Vol. 10478:
Molecular-Guided Surgery: Molecules, Devices, and Applications IV
Brian W. Pogue; Sylvain Gioux; Greg Biggs, Editor(s)

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