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Proceedings Paper

Feature characterization of scarring and non-scarring types of alopecia by multiphoton microscopy (Conference Presentation)
Author(s): Inga Saknite; Manuel Valdebran; Jessica Lin; Griffin R. Lentsch; Joshua N. Williams; Mihaela Balu; Bruce J. Tromberg; Natasha A. Mesinkovska

Paper Abstract

Treatment and management of alopecia are highly determined by an accurate diagnosis, which can be challenging due to the lack of methods to properly visualize hair follicles. Current standard diagnosis is based on dermoscopy for non-scarring alopecia and scalp biopsy for scarring types of alopecia. Dermoscopy can be inconclusive, while biopsy is a painful procedure. In this study, we used a clinical tomograph based on multiphoton microscopy (MPM) to non-invasively image the scalp of 5 healthy subjects and of 12 patients affected by non-scarring alopecia (androgenetic and areata) and scarring alopecia (frontal fibrosing). MPM is capable of non-invasive in vivo imaging of follicular structures in human scalp including hair shafts, hair follicles and sebaceous glands via two-photon excited fluorescence (TPEF) from keratin and NADH/FAD and of the papillary dermis surrounding the hair follicles through second harmonic generation (SHG) from collagen and TPEF from elastin fibers. In normal and non-scarring alopecia patients, MPM often identified presence of sebaceous glands associated with hair follicles, while MPM images of scarring alopecia were characterized by miniaturization of hair follicles as well as by presence of macrophages and lymphocytes surrounding hair follicles. A quantitative analysis involving measurement of hair follicle diameter sizes showed they were significantly smaller in scarring comparing to non-scarring alopecia patients and to normal scalp subjects (p < 0.043). This study shows, in a limited number of patients, that MPM imaging can non-invasively identify morphological features that distinguish scarring from non-scarring alopecia.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 104670I (14 March 2018); doi: 10.1117/12.2291410
Show Author Affiliations
Inga Saknite, Beckman Laser Institute and Medical Clinic (United States)
Institute of Atomic Physics and Spectroscopy, Univ. of Latvia (Latvia)
Manuel Valdebran, Univ. of California, Irvine (United States)
Jessica Lin, Univ. of California, Irvine (United States)
Griffin R. Lentsch, Beckman Laser Institute and Medical Clinic (United States)
Joshua N. Williams, Beckman Laser Institute and Medical Clinic (United States)
Mihaela Balu, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Natasha A. Mesinkovska, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 10467:
Photonics in Dermatology and Plastic Surgery 2018
Bernard Choi; Haishan Zeng, Editor(s)

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