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Detection of changes in bone quality of osteoporotic model induced by sciatic nerve resection by using Raman spectroscopy
Author(s): Yasumitsu Ishimaru; Yusuke Oshima; Yuuki Imai; Tadahiro Iimura; Sota Takanezawa; Kazunori Hino; Hiromasa Miura
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Paper Abstract

To detect the bone quality loss in osteoporosis, we performed Raman spectroscopic analysis of sciatic nerve resection (NX) mice. Eight months after surgery, lower limbs were collected from the mice and fixed with 70% ethanol. Raman spectra of anterior cortical surface of the proximal tibia at 5 points in each bone were measured by RENISHAW inVia Raman Microscope. Excitation wave length was 785 nm. We also performed DXA and micro CT measurement to confirm the bone mineral density and bone microstructure in the osteoporotic model induced by sciatic nerve resection. In the result of Raman spectroscopy, we detected changes of Raman peak intensity ratio in carbonate/phosphate, mineral/combined proline and hydroxyproline and mineral/phenylalanine. In addition, in the result of micro CT, we found significant changes in VOX BV/TV, Trabecular number, thickness, cancellous bone mineral density, cortical thickness and cortical bone mineral density. The results suggest that not only the bone mineral density but also bone quality reduced in the NX mice. We conclude that Raman spectroscopy is a useful for bone quality assessment as a complementary technique for conventional diagnostics.

Paper Details

Date Published: 20 February 2018
PDF: 4 pages
Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 1049722 (20 February 2018); doi: 10.1117/12.2291258
Show Author Affiliations
Yasumitsu Ishimaru, Ehime Univ. Graduate School of Medicine (Japan)
Yusuke Oshima, Ehime Univ. Hospital (Japan)
Yuuki Imai, Ehime Univ. (Japan)
Tadahiro Iimura, Ehime Univ. (Japan)
Sota Takanezawa, Ehime Univ. Graduate School of Medicine (Japan)
Kazunori Hino, Ehime Univ. Graduate School of Medicine (Japan)
Hiromasa Miura, Ehime Univ. Graduate School of Medicine (Japan)


Published in SPIE Proceedings Vol. 10497:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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