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Proceedings Paper

Particle tracking and extended object imaging by interferometric super resolution microscopy
Author(s): Itay Gdor; Seunghwan Yoo; Xiaolei Wang; Matthew Daddysman; Rosemarie Wilton; Nicola Ferrier; Mark Hereld; Oliver (Ollie) Cossairt; Aggelos Katsaggelos; Norbert F. Scherer
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Paper Abstract

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.

Paper Details

Date Published: 23 February 2018
PDF: 9 pages
Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049911 (23 February 2018); doi: 10.1117/12.2291245
Show Author Affiliations
Itay Gdor, The Univ. of Chicago (United States)
Seunghwan Yoo, Northwestern Univ. (United States)
Xiaolei Wang, The Univ. of Chicago (United States)
Matthew Daddysman, The Univ. of Chicago (United States)
Rosemarie Wilton, Argonne National Lab. (United States)
Nicola Ferrier, Argonne National Lab. (United States)
Mark Hereld, Argonne National Lab. (United States)
Oliver (Ollie) Cossairt, Northwestern Univ. (United States)
Aggelos Katsaggelos, Northwestern Univ. (United States)
Norbert F. Scherer, The Univ. of Chicago (United States)


Published in SPIE Proceedings Vol. 10499:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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