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Proceedings Paper

Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells
Author(s): Björn Kemper; Lena Kastl; Jürgen Schnekenburger; Steffi Ketelhut
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Paper Abstract

Main restrictions of using laser light in digital holographic microscopy (DHM) are coherence induced noise and parasitic reflections in the experimental setup which limit resolution and measurement accuracy. We explored, if coherence properties of partial coherent light sources can be generated synthetically utilizing spectrally tunable lasers. The concept of the method is demonstrated by label-free quantitative phase imaging of living pancreatic tumor cells and utilizing an experimental configuration including a commercial microscope and a laser source with a broad tunable spectral range of more than 200 nm.

Paper Details

Date Published: 23 February 2018
PDF: 6 pages
Proc. SPIE 10503, Quantitative Phase Imaging IV, 1050313 (23 February 2018); doi: 10.1117/12.2291071
Show Author Affiliations
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)
Lena Kastl, Westfälische Wilhelms-Univ. Münster (Germany)
Jürgen Schnekenburger, Westfälische Wilhelms-Univ. Münster (Germany)
Steffi Ketelhut, Westfälische Wilhelms-Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 10503:
Quantitative Phase Imaging IV
Gabriel Popescu; YongKeun Park, Editor(s)

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