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Proceedings Paper

Diffraction-based BioCD biosensor for point-of-care diagnostics
Author(s): H. Choi; C. Chang; C. Savran; D. Nolte
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Paper Abstract

The BioCD platform technology uses spinning-disk interferometry to detect molecular binding to target molecular probes in biological samples. Interferometric configurations have included differential phase contrast and in-line quadrature detection. For the detection of extremely low analyte concentrations, nano- or microparticles can enhance the signal through background-free diffraction detection. Diffraction signal measurements on BioCD biosensors are achieved by forming gratings on a disc surface. The grating pattern was printed with biotinylated bovine serum albumin (BSA) and streptavidin coated beads were deployed. The diameter of the beads was 1 micron and strong protein bonding occurs between BSA and streptavidin-coated beads at the printed location. The wavelength for the protein binding detection was 635 nm. The periodic pattern on the disc amplified scattered light into the first-order diffraction position. The diffracted signal contains Mie scattering and a randomly-distributed-bead noise contributions. Variation of the grating pattern periodicity modulates the diffraction efficiency. To test multiple spatial frequencies within a single scan, we designed a fan-shaped grating to perform frequency filter multiplexing on a diffraction-based BioCD.

Paper Details

Date Published: 20 February 2018
PDF: 4 pages
Proc. SPIE 10501, Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics, 105011K (20 February 2018); doi: 10.1117/12.2291069
Show Author Affiliations
H. Choi, Purdue Univ. (United States)
C. Chang, Purdue Univ. (United States)
C. Savran, Purdue Univ. (United States)
D. Nolte, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 10501:
Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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