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Proceedings Paper

Multiphoton microscopy of ECM proteins in baboon aortic leaflet
Author(s): Mariacarla Gonzalez; Ilyas Saytashev; Camila Luna; Brittany Gonzalez; Alejandro Pinero; Manuel Perez; Sharan Ramaswamy; Jessica Ramella-Roman
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Paper Abstract

The extracellular matrix (ECM) plays crucial role in defining mechanical properties of a heart valve yet the mechanobiological role of the ECM proteins – collagen and elastin - in living heart valve leaflets is still poorly understood. In this study, non-linear microscopy was used to obtain three dimensional images of collagen and elastin arrangement in aortic leaflets under combined steady flow (850 ml/min) and cyclic flexure (1 Hz) mechanical (dynamic) training. A novel bioreactor capable of mimicking the flow conditions in a living heart was used in this study and was optimized for microscopic imagery. A custom made non-linear microscope was used in this study to provide Second Harmonic Generation (SHG) imaging of collagen arrangement and two-photon imaging of elastin. Two control and three trained leaflet samples from static and dynamic tissue culture were imaged to observe protein changes in the tissue for a period of seven days. Dynamic training led to a decrease in alignment index of the protein fibers compared to the static treatment.

Paper Details

Date Published: 22 February 2018
PDF: 7 pages
Proc. SPIE 10471, Diagnostic and Therapeutic Applications of Light in Cardiology 2018, 104710Y (22 February 2018); doi: 10.1117/12.2291024
Show Author Affiliations
Mariacarla Gonzalez, Florida International Univ. (United States)
Ilyas Saytashev, Florida International Univ. (United States)
Camila Luna, Florida International Univ. (United States)
Brittany Gonzalez, Florida International Univ. (United States)
Alejandro Pinero, Florida International Univ. (United States)
Manuel Perez, Florida International Univ. (United States)
Sharan Ramaswamy, Florida International Univ. (United States)
Jessica Ramella-Roman, Florida International Univ. (United States)

Published in SPIE Proceedings Vol. 10471:
Diagnostic and Therapeutic Applications of Light in Cardiology 2018
Guillermo J. Tearney; Kenton W. Gregory; Laura Marcu, Editor(s)

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