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Statistical inference in single molecule measurements of protein adsorption
Author(s): Megan J. Armstrong; Stanislav Tsitkov; Henry Hess
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Paper Abstract

Significant effort has been invested into understanding the dynamics of protein adsorption on surfaces, in particular to predict protein behavior at the specialized surfaces of biomedical technologies like hydrogels, nanoparticles, and biosensors. Recently, the application of fluorescent single molecule imaging to this field has permitted the tracking of individual proteins and their stochastic contribution to the aggregate dynamics of adsorption. However, the interpretation of these results is complicated by (1) the finite time available to observe effectively infinite adsorption timescales and (2) the contribution of photobleaching kinetics to adsorption kinetics. Here, we perform a protein adsorption simulation to introduce specific survival analysis methods that overcome the first complication. Additionally, we collect single molecule residence time data from the adsorption of fibrinogen to glass and use survival analysis to distinguish photobleaching kinetics from protein adsorption kinetics.

Paper Details

Date Published: 20 February 2018
PDF: 8 pages
Proc. SPIE 10506, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV, 105061A (20 February 2018); doi: 10.1117/12.2290918
Show Author Affiliations
Megan J. Armstrong, Columbia Univ. (United States)
Stanislav Tsitkov, Columbia Univ. (United States)
Henry Hess, Columbia Univ. (United States)

Published in SPIE Proceedings Vol. 10506:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV
Alexander N. Cartwright; Dan V. Nicolau; Dror Fixler, Editor(s)

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