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Proceedings Paper

Cerebral oxygenation and blood flow distributions along the capillary path in awake mice (Conference Presentation)
Author(s): Baoqiang Li; Ikbal Sencan; Tatiana V. Esipova; Kivilcim Kilic; Mohammad Moeini; Mohammad A. Yaseen; Buyin Fu; Sreekanth Kura; Frédéric Lesage; Sergei A. Vinogradov; Anna Devor; David A. Boas; Sava Sakadžić
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Paper Abstract

Cortical capillary blood flow and oxygenation are highly heterogeneous. Mapping absolute capillary blood flow and oxygenation along capillary path is a key step towards understanding how oxygen is transported and delivered in a complex microvascular network to enable adequate tissue oxygenation. In this work, we applied two-photon microscopic imaging of intravascular oxygen partial pressure (PO2) to measure both oxygen concentration and red blood cell (RBC) flux in cortical arterioles, capillaries, and venules. Imaging was performed in awake, head-restrained C57BL/6 mice (n=15), through a chronic sealed cranial window centered over the E1 whisker barrel. We obtained a detailed mapping of the resting state cortical microvascular PO2 in all arterioles and venules, and both PO2 and RBC flux in most capillaries down to 600 μm depth from the cortical surface (n=6,544 capillaries across all mice). Capillary RBC speed and density were also extracted and all measurements were co-registered with the microvascular angiograms. We characterized the distributions of capillary PO2 and flow as a function of branching order and cortical depth. The results show strong positive correlation between oxygenation and flow in the capillary segments, with an increased correlation in downstream capillaries. We have also observed homogenization of both oxygenation and flow in deeper cortical layers, which may imply a mechanism to improve oxygen delivery without increasing global blood flow in the area with increased metabolism.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10481, Neural Imaging and Sensing 2018, 1048109 (14 March 2018); doi: 10.1117/12.2290858
Show Author Affiliations
Baoqiang Li, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Ikbal Sencan, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Tatiana V. Esipova, Univ. of Pennsylvania (United States)
Kivilcim Kilic, Univ. of California, San Diego (United States)
Mohammad Moeini, Institute of Biomedical Engineering, Ecole Polytechnique de Montréal (Canada)
Mohammad A. Yaseen, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Buyin Fu, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Sreekanth Kura, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Frédéric Lesage, Institute of Biomedical Engineering, Ecole Polytechnique de Montréal (Canada)
Sergei A. Vinogradov, Univ. of Pennsylvania (United States)
Anna Devor, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Univ. of California, San Diego (United States)
Harvard Medical School (United States)
David A. Boas, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Sava Sakadžić, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States)
Harvard Medical School (United States)


Published in SPIE Proceedings Vol. 10481:
Neural Imaging and Sensing 2018
Qingming Luo; Jun Ding, Editor(s)

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